This past week, LCGC International published a variety of articles on hot topics in separation science. We discuss how mass spectrometry (MS) unveiled new insights into how music modulates protein networks in lipid nanoparticle delivery and how gas chromatography (GC) can be sed to identify aroma types in unburnt cannabis. Also, we make a call for nominations for the 2026 HTC Innovation Award.

This is the Best of the Week.

GC–MS Analysis of Time-Dependent Flavor Development in Marinade Systems

Researchers from Jiangxi Normal University, China Agricultural University, and Hunan Xiweijia Biotechnology studied how cooking time affects the flavor and quality of Chinese-style marinades using headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) (1). This study, which was published in the journal Current Research in Food Science, identified 246 volatile compounds, with 29 key contributors linked to sensory characteristics (1). Flavor intensity peaked at 40–60 minutes of cooking, while longer times caused aroma loss (1). A 40-min stewing time achieved optimal flavor balance and antioxidant activity. The findings reveal how stewing time influences amino acid and fatty acid dynamics, offering guidance for optimizing traditional marination processes.

Pink Floyd and the Blood–Brain Barrier: Mass Spectrometry Explores How Music Modulates Protein Networks in LNP Delivery

A multinational research team discovered that audible sound waves, specifically Pink Floyd’s “Another Brick in the Wall, Part 1,” can enhance lipid nanoparticle (LNP) uptake and brain penetration. Published in the Journal of Controlled Release, the study found that low-frequency sounds (10–250 Hz) increased neuronal LNP uptake and boosted gene expression tenfold in cultured neurons (2). In mice, sound exposure enhanced mRNA expression in the midbrain and thalamus, while MS revealed modulation of proteins linked to cellular uptake (2). This proof-of-concept suggests music could noninvasively improve drug delivery to the brain, offering new potential for treating neurological diseases.

Establishing a Cannabis Aroma Lexicon through Gas Chromatographic and Sensory Analysis

In this study, a team of researchers from Oregon State University and Dow Neurobiology developed the first standardized aroma lexicon for cannabis inflorescence. This study was published in PLOS One (3). Using GC and sensory analysis, they identified 25 descriptive aroma terms to classify unburnt cannabis. Human panel evaluations of 91 samples revealed that Type I (high THC) cannabis had skunky, musty, and animalic aromas, while Type III (high CBD) exhibited citrus and fruity notes (3). However, terpene and sulfur compound profiles alone could not reliably predict aroma perception. The study provides a foundational framework for future research linking chemical composition, sensory attributes, and consumer quality perceptions in cannabis (3).

Driving the Spirit of Innovation: The 2026 HTC Innovation Award

LCGC International, in collaboration with the HTC-19 Conference Scientific Committee, has announced the 2026 HTC Innovation Award to honor a separation scientist who has introduced pioneering methodologies, instrumentation, or techniques with societal impact. The award reflects MJH Life Sciences’ core value of innovation, part of its “Driving Spirit” (4). HTC-19, which will take place from May 26 to 29, 2026, in Leuven, Belgium, continues a legacy begun in 1990 as a premier forum for advances in separation science (4). Previous winners include Carolin Huhn, Ryan Kelly, Szabolcs Fekete, and Bob Pirok. Nominations are open worldwide to scientists within 15 years of earning their PhD.

Mass Spectrometry Characterization of HIV Broadly Neutralizing Antibody PGT 121

Researchers at the University of Buffalo used combined middle-up and bottom-up liquid chromatography–tandem mass spectrometry (LC–MS/MS) to comprehensively characterize the HIV-1 broadly neutralizing antibody PGT 121. Their study, discussed with LCGC International by corresponding author Troy Wood, identified multiple Fc and site-specific glycoforms—mainly G0F and G1F—and an unexpected N-terminal serine, revealing significant structural heterogeneity (5). By integrating both LC–MS/MS workflows, the team achieved detailed insight into post-translational modifications (PTMs), confirming glycosylation sites and enhancing understanding of antibody proteoforms (5). This dual approach supports improved quality control, pharmacokinetic interpretation, and clinical development of therapeutic antibodies targeting HIV-1.

1. Chasse, J. GC–MS Analysis of Time-Dependent Flavor Development in Marinade Systems. LCGC International. Available at: https://www.chromatographyonline.com/view/gc-ms-analysis-of-time-dependent-flavor-development-in-marinade-systems (accessed 2025-11-6).
2. Chasse, J. Pink Floyd and the Blood–Brain Barrier: Mass Spectrometry Explores How Music Modulates Protein Networks in LNP Delivery. LCGC International. Available at: https://www.chromatographyonline.com/view/pink-floyd-and-the-blood-brain-barrier-mass-spectrometry-explores-how-music-modulates-protein-networks-in-lnp-delivery (accessed 2025-11-6).
3. Chasse, J. Establishing a Cannabis Aroma Lexicon through Gas Chromatographic and Sensory Analysis. LCGC International. Available at: https://www.chromatographyonline.com/view/establishing-a-cannabis-aroma-lexicon-through-gas-chromatographic-and-sensory-analysis (accessed 2025-11-6).
4. Matheson, A. Driving the Spirit of Innovation: The 2026 HTC Innovation Award. LCGC International. Available at: https://www.chromatographyonline.com/view/driving-the-spirit-of-innovation-the-20226-htc-innovation-award (accessed 2025-11-6).
5. Chasse, J. Mass Spectrometry Characterization of HIV Broadly Neutralizing Antibody PGT 121. LCGC International. Available at: https://www.chromatographyonline.com/view/mass-spectrometry-characterization-of-hiv-broadly-neutralizing-antibody-pgt-121 (accessed 2025-11-6).